Lead Acetate-induced Changes in Haematological Indices and Bone Marrow of Adult Wistar Rats: Protective Role of α-Tocopherol (Vitamin E)

Abstract

Lead exposure has been associated with several adverse health effects involving multiple body systems and organ failure. Hence, the objective of this paper was to the effects of α-Tocopherol (Vitamin E) on haematological indices and bone marrow of lead acetate (LA)-exposed adult Wistar rats. Twenty (20) adult Wistar rats (n=5) were randomly assigned as follows: Control group (A) received 1ml of distilled water; Group B received 100 mg/kg body weight (BW) of LA; Group C received 50 mg/kg BW of α-Tocopherol and 100 mg/kg BW of LA; Group D received 50 mg/kg BW α-Tocopherol only. All administrations, via an oral gavage, lasted for twenty-eight days. Following the sacrifice of experimental rats, blood samples were collected in Ethylenediaminetetraacetic acid bottles for hematological analysis and the femur of rats were fixed in 10% neutral buffered formalin for histological evaluation. Results showed that the haematological indices in group B rats were significantly different (P<0.05) when compared to control. Also, the histological findings in group B rats revealed mild hypoplasia of the haematopoietic cells with severe vacuolations following comparison to control. However, in group C rats treated with α-Tocopherol, the adverse effects of LA were attenuated. Rats in group D showed no significant differences (P>0.05) in the parameters investigated when compared to control. Conclusively, results from this study showed that α-Tocopherol can mitigate the toxic effects of lead on the haematological system, and further studies are needed to corroborate these findings and investigate the mechanisms of action.