Cytotoxicity and Anti-Diabetic Properties of Leaves of Some Medicinal Plants collected around Zaria, Kaduna State Nigeria

Abstract

The aim of this study is to establish the anti-diabetic potential of Blighia unijugata Baker, Anthocleista djalonensis, Senna sieberiana, Senna singueana and Ficus exasperate. Blighia unijugata Baker, Anthocleista djalonensis, Senna sieberiana, Senna singueana Delile and Ficus exasperate Vahl were extracted with methanol and fractionated using petroleum ether, chloroform and ethylacetate. The phytochemical analysis, cytotoxicity and antidiabetic properties of the fractions were investigated using standard methods. Phytochemical results of the plants fractions showed various amount of alkaloids ranging from 1.2 - 4.7%with moderate amount of flavonoids, steroids, saponins and trace amount of tannins. The results of cytotoxicity using Fundulopachas gadneri showed senna singueana Delile, ethylacetate fraction and Blighia unijugata  Baker- pet ether fraction being the most active (LC₅₀ 5. 23). Antidiabetic results showed that most of the fractions are highly active reducing the sugar level of the induced albino rats to normal with Ficus exasperate Vahl, ethylacatate fraction and Blighia unijugata Baker-chloroform fraction showing higher activity against all the induced rats. Comparatively the two fractions showed higher activities than the standard drugs. The results of this research therefore showed  Ficus exasperate Vahl, ethylacatate fraction and Blighia unijugata Baker-chloroform fraction possess great potentials as anti-diabetic agents with animals treated withBlighia unijugata Baker-chloroform fraction having better recovery because the experimental animals had steady weight gain and could therefore be a potential as anti-diabetic drugs.