Evaluation of Comparative Performance of Xylene and Fresh Lemon Fruits Extract as Dewaxing Agent in Histopathology Staining for Liver, Kidney, and Lung in Sectioned Wistar Rats

Abstract

Xylene is a significant material in histopatholoy staining, however, it poses health risks to people and therefore, the histopathologist requires a substitute which could be less costly with zero or to minimal health risk if ingested, inhaled or in direct contact with eyes or skin. Therefore, the objective of this paper is to evaluate the comparative performance of xylene and fresh lemon fruits extract as dewaxing agent in histopathology staining for liver, kidney, and lung in sectioned Wistar rats using appropriate standard techniques. Data obtained from both lemon water and using Haematoxylin and Eosin (H&E) and a photomicrograph revealed that concentrated lemon water could serve as a bio-safe substitute for xylene in deparaffinization process during sectioning.