Protective Effects of Trans-Ferulic acid on Tamoxifen Induced Heptatic and Renal Oxidative Stress in Male Wister Albino Rat

Abstract

This study was designed to assess the protective effects of trans-ferulic acid on tamoxifen induced hepatic and renal oxidative stress in male Wister albino rates using appropriate standard methods. Twenty four male albino rats were randomly divided into 4 (four) groups of 6 (six) animals each. Group A, B, C and D where group A is control animals group B and C animals were treated with 0.4 mg/kgof tamoxifen and100 mg/kg body weight of trans-ferulic acid respectively. Group D animals were simultaneously administered with 100 mg/kg of trans-ferulic acid and 0.4 mg/kg of tamoxifen for twenty one days. UV/VIS spectrophotometer was used for the analysis of indicators. A significant elevation was observed in plasma bilirubin, urea and creatinine by tamoxifen treated group with mean concentrations of 35.50 ± 1.8 mg/dl, 2.15±0.06 mg/dl and 7.80 ±9.29 mg/dl respectively, when observed with mean concentrations of control group 14.7±0.31 mg/dl, 0.98±0.05 mg/dl and 3.31±0.15 mg/dl. While the mean concentrations of trans-ferulicacid treatment were 21.2±0.36 mg/dl,1.07±0.03 and 3.65 mg/dl, significantly protected on the effects of the tamoxifen- induced. ALP, AST, ALT activities were also increased significantly (p<0.05) on mean concentration of 65±1.0 U/L, 11.08±0.12 U/L and 678.20±13.30 U/L in the treated group by tamoxifen respectively when viewed with the control (p<0.05) and trans-ferulic acid treatment reduced the increased enzyme activities by mean concentrations of 43.6±1.0, 5.54±0.26 U/L and. Furthermore, tamoxifen administration decreased the level of hepatic superoxide dismutase (SOD), and catalase (CAT) activities by 32.5±2.30 µmole and 0.50±0.05µmole respectively when viewed with mean concentration of the control (49.0±1.15µmole and 1.37±0.02µmole) and trans-ferulic acid treatments significantly ameliorated the decreased in the hepatic enzymes activities (P<0.05) with mean valued 44.0±1.40 and 1.26 ± 0.05 µmole. Also the level of hepatic ascorbic acid was decreased significantly on treatment with tamoxifen by 41.7±2.9 U/L when compared with the mean concentration of the control (60.48±1.45 U/L), while treatment with trans-ferulic acid protected against the decreased level of ascorbic acid by 55.44±1.76 U/L. Moreover administration of tamoxifen caused a significant (P<0.05) increase in MDA by (97.9±6.9 U/L), while treatment with trans-ferulic acid protected against increased in the level of MDA to (22±0.70 U/L) .These study suggested that trans-ferulic acid reduced the effect of liver and kidney toxicity and induced stress posed by tamoxifen in rats.