Histology of Liver of Claria gariepinus Fingerlings Fed with Spent Sorghum from Locally Fermented Drink (Burukutu)

Abstract

High fiber content, increased presence of carbohydrates, anti-nutritional factors can have adverse effects on the digestive system of fish and therefore on fitness, health and production capacity. A 70-day study was conducted to evaluate the histological alteration of the liver of Clarias gariepinus fed with spent sorghum. Ten isonitrogenous diets were formulated. Spent sorghum was added to the diet to replace maize at graded levels; 0%, 10%, 20%, 30%, 40%, 50%, 70%, 80%, 90% and 100%. Total of 360 fish (8.330±0.00g) were randomly assigned to the five treatment diets. Each treatment contained 10 fish per bowls and each treatment was triplicated in a completely randomized design (CRD). Fish were fed twice daily at 5% body weight in equal proportions. At the end of the feeding trials, the liver of the fish were taken for histological analysis. The histological results of the liver showed that some treatments showed cytoplasm with irregular arrangement and dislocated nucleus; slight haemorrhage in the hepatic parenchyma with peribular capsule indistinct between hepatocytes and enlarged hepatocytes which is not a dietary treatment related.