Effect of Substrate Concentration and Temperature for Bio-Enzyme Production Using Enterococcus faecalis Isolated from Waste Dump Soil in Sokoto Metropolis, Nigeria

Abstract

The study evaluated the effect of substrate concentration and temperature on the production of amylase enzyme by Enterococcus faecalis from the wastes dump soil Sokoto Metropolis, Nigeria. Five bacteria species were isolated and biochemically characterized which include E. coli, B. subtilis, S. aureus, E. faecalis and P. aeroginosa. The screening of identified bacteria for amylase production was carried out and all bacteria produced a wide halo on a starch agar. E. faecalis produced highest zone of clearance with 70mm,B.subtilis 50mm,S.aureus 34mm, E. coli 29mm,thenB.cereus with the  least zone of clearance of 24mm. proximate analysis of the potato peels sample shows that the potatoes peels has high carbohydrate content with value of 75.737 recorded. The effect of substrate concentration was studied at 5g,10g,15g and 20g. The highest amylase production was recorded (20g) with value of 1.218 and 15g with the least absorbance value of 0.973.The effect of temperature were studied at 25˚C, 35˚C, 45˚C, and 55˚C. The temperature of 45˚C has the highest absorbance value of 1.691. While the least was recorded at55˚C with value of 0.970.These shows that temperature and substrate concentration has a vital role to play in the production of microbial enzymes. It’s concluded in this study that waste dump site is a reservoir of important microorganisms and potato peels is a good substrate for enzyme production and therefore can be utilized instead of dumping it for natural degradation.