Changes in Oxidative Stress Parameters in Wistar Rats Administered Soft Drink and Menthol Candy

Abstract

The present study aimed to evaluate changes of stress oxidative enzymes and electrolytes of wistar rats administered soft drink and menthol candy. Seventy Wistar rats of weight between 125g and 175g were utilized for the study. The rats were allowed to acclimatize for seven days. The rats were divided into 10 groups of seven rats as follows: Group 1: normal control, Group 2, 3 and 4 were given 8ml, 5 and 2.5ml/kg body weight lacasara. Group 5, 6 and 7 were given 0.3g, 0.22g and 0.11g/kg body weight tom-tom candy. Group 8, 9 and 10 were administered 0.34g in 8ml, 0.22g in 5ml and 0.11g in 2.5ml/kg body weight of tom-tom mix with lacasara respectively. Treatment with tom-tom, lacasara and tom-tom mix with lacasara at various doses was carried out for 42 days. Electrolyte, creatinine and urea in the serum, superoxide dismutase (SOD), catalase (CAT) and malonyldialdehyde (MDA) in liver and kidney ware determined. The results showed significant increase in serum Na⁺, K⁺, Cland LPO, decrease in SOD, and CAT, in the liver and kidney of groups 2, 3, 5, 6, 8, 9 and 10 compared to control. Furthermore, significant decrease was observed in LPO and increase SOD and CAT of group 7 in the liver and kidney compared to group 5 and 6. There were no significant difference in creatinine and urea in the serum of group 7 compared to group 5 and 6. In conclusion, chronic consumption of tom-tom, lacasara or tom-tom mix with lacasara induced oxidative stress. This effect was confirmed by kidney histological study, showing inflammation of proximal tubule and tubular cells.