Identification and Antibiotic Susceptibility Pattern of Enterobacteriaceae Isolated from Gecko (Hemidactylus frenatus)

Abstract

Diseases and infections which are naturally transmitted between animals and humans are of major concern worldwide. Geckos (Hemidactylus frenatus) are known to be potential reservoirs of many zoonotic enteropathogens. This study was designed to isolate, identify, and evaluate the antibiotic susceptibility pattern of Enterobacteriaceae from Geckos. Using standard microbiological procedures, bacteria were isolated from 138 intestinal samples of Hemidactylus frenatus collected from different sampling sites. A total of 20 bacterial species of 9 different genera were identified using automated Colorimetry VITEK 2 system. The percentage occurrences were Enterobacter aerogenes (35%), Proteus mirabilis (15%), Salmonella ser paratyphi B (10%), Serratia fonticola (10%), Enterobacter kobei (10%), Raoultella ornithinolytica (5%), Sphingomonas paucimobilis (5%), Acinetobacter baumannii, (5%) and Burkholderia cepacia (5%). Results obtained from the antibiotic susceptibility pattern according to CLSI guidelines revealed that all the 20 bacterial species have varying rate of resistance with 20 (100%) showing resistance to Ciprofloxacin (CPX), 20 (100%) Pefloxacin (PEF), 19 (95%), Augmentin (AU), 11 (55%) Cotrimoxazole (CXT), 10 (50%) Streptomycin (S), 9 (45%) Chloramphenicol (CH), 6 (30%) Gentamycin (CN), 3 (15%) Ofloxacin (OFX). This study revealed that Enterobacteriaceae in the intestine of Geckos are multidrug resistant and are potentially harmful when in contact directly or indirectly with humans. It becomes important to educate people on the importance of personal hygiene in order to eradicate Geckos from our environment.