Dominant Bacterial and Archaeal Phyla Associated with Top Soils Sourced from Commercial Farm Holding in Delta State, Nigeria

Abstract

The dominant bacteriological and archaeal phyla of compounded soils  sourced from a commercial farm estate located in Amukpe town and a nearby control in Adavware community both in Delta State, were evaluated with the aid of Next Generation Sequencing (NGS) protocols. The residual herbicide and pesticide composition of the bulked soils were also  determined using gas chromatography (GC) and electron capture detector (ECD). Total concentrations of the extracted DNA were 6.83 and 6.65 ng/μl for the control and experimental soils. Nine (9) bacterial phyla;  Proteobacteria, Actinobacteria, Chloroflexi, Firmicutes, Verrucomicrobia, Planctomycetes, Bacteroidetes Acidobacteria, and Elusimicrobia were  observed in the control soil. Thirteen (13) bacterial phyla; Elusimicrobia, Fibrobacteres Lentisphaerae, Armatimonadetes, Cyanobacteria/Chloroplast, Bacteroidetes, Actinobacteria, Proteobacteria, Chloroflexi, Firmicutes, Acidobacteria,  Planctomycetes and Verrucomicrobia were detected in the experimental soil. Two (2) archaeal phyla; Euryarchaeota, and Diapherotrites were detected both the experimental and control soil, whilst an additional archaeal phylum; Woesearchaeota was present in only the experimental soil. The total organochloride phosphate component of the experimental soil was 1.4μg/Kg and 0.4μg/Kg for the control soil  respectively.

Keywords: Amukpe, Next Generation Sequencing, Adavware, bacterial and archaeal phyla