Evaluation of biofilm and enterotoxin producing capacity of methicillin-resistant Staphylococcus aureus isolated from healthy persons

Abstract

This study is aimed at investigating biofilm and enterotoxin producing capacity of methicillin-resistant Staphylococcus aureus isolated from nostrils of healthy students of Delta State University Oleh campus. Three hundred (300) nasal samples were collected using sterile swab stick and the isolates were identified using relevant biochemical tests. Determination of methicillin-resistant Staphylococcus aureus was carried out with the aid of Oxacillin sensitivity disc to test the presence of penicillin binding protein2a (PBP2a). The Methicillin-resistant Staphylococcus aureus (MRSA) isolates were screened to determine their biofilm producing ability using crystal binding assay. Latex agglutination test was carried out to detect the presence of enterotoxins among the MRSA isolates. The incidence of MRSA colonization among healthy individuals in the community of the S. aureus isolated was 47 (48.9%). Enterotoxins B was mostly detected, only three Enterotoxin C were detected. All the MRSA isolates tested for the presence of biofilm showed positive results. It has been observed that MRSAs are capable of producing biofilms and enterotoxins which pose the risk antibiotic resistance and human Staphylococci toxicity.

Keywords: Enterotoxins, Biofilm, Staphylococcus aureus