Hormonal Profiling in Ungerminated and Germinated Seeds of African Yam Bean (Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms)

Abstract

Hormonal profiles of ungerminated and germinated seeds of Sphenostylis stenocarpa were studied. The hormone gibberellin (GA) was extracted with 80% methanol. The hormone was purified with polyvinyl pyrrolidone (PVP) column chromatography and quantified with high performance liquid chromatography (HPLC). High performance liquid chromatography analysis identified five endogenous gibberellins GA₁, GA₃, GA₄, GA₇ and GA₂₀. Quantification of gibberellin in ng/g (dw) for ungerminated and germinated seeds showed that GA₁ was highest in quantity than GA₃, GA₄, GA₇ and GA₂₀ in all the fractions in ungerminated seed while GA₃ was highest in quantity in germinated seed than GA₁, GA₄, GA₇ and GA₂₀. This study suggests that hormone extracted from ungerminated and germinated seeds of S. stenocarpa could be a source of phytohormones for research and agricultural purposes

Keywords: Hormonal Profile, Ungerminated, Germinated, Sphenostylis stenocarpa