Blocking actions of the sea anemone, Bunodosoma cavernata extract on Histamine and Acetylcholine induced contractile actions of the wild grasscutter and rabbit Ilea

Abstract

Extract from the sea anemone, Bunodosoma cavernata was prepared by homogenizing 100 of the sea anemones in 300ml of 0.9% saline in a Moulinex blender. This was centrifuged at 4000 revolutions per minute for 10 minutes. The supernatant was re – centrifuged to obtain the crude extract. The extract (1ml/l) was found to elicit long lasting contractions on the ilea of wild rabbit and grass cutter. It was also found to block acetylcholine (ACh) – induced contractions of the ileum of grass cutter (90 + 6.2% inhibition + SEM) and the contractile action induced by ACh (0.2mg and 0.4mg) (89 + 5.8% inhibition + SEM) and histamine (0.075mg and 0.15mg) on the rabbit ileum (80 + 4.6% inhibition + SEM) . The antagonism by the extract was non – selective.


(Journal of Applied Sciences & Environmental Management: 2002 6(2): 11-16)