Bacteriological quality and antibiogram of isolates from potable water sources in ekosodin community, Benin City, Nigeria

Abstract

This study sought to determine the bacteriological quality of some boreholes and storage tanks used for drinking and other domestic purposes in Ekosodin community and evaluated the antimicrobial susceptibility patterns of the groundwater resources isolates. A total of 48 samples were collected from three boreholes and storage tanks for a period of two months. Samples were analyzedfor microbiological content following standard procedures. Feacal Coliform (FC) and Feacal Streptoccocci (FS) counts and antimicrobial susceptibility patterns were evaluated using the Most Probable Number and Kirby-Bauer methods respectively.The mean FC counts (1.2±0.3Cfu/100ml - 55±10Cfu/100ml) and meanSF counts (0cfu/100ml – 9.25±1.1Cfu/100ml) in some of the samples examined, exceeded the WHO limit of 0Cfu/100ml for drinking water. Significant differences were recorded for mean FS counts betweensome boreholes and storage tanks. Escherichia coli (34%), Klebsiella sp (23%), Pseudomonas sp (19%) and Feacal Streptococci(12%) were amongst the identified isolates from the water samples. The antibiogram analysis revealed that all isolates (87%) except some strains of Feacal Streptococci were sensitive to ciprofloxacin and 73% of isolates were resistant to Erythromycin. All isolates exhibited a multi drug resistance to at least eight antibiotics. The microbial analysis revealed a faecal contamination of the groundwater, making it unfit for drinking without proper disinfection.

Keywords: Ciprofloxacin, Multidrug resistance, Feacal Coliforms, Feacal Streptococci, Erythromycin