Comparison of haematological indices, blood group and genotype of Chrysicthys nigrodigtatus and Synodontis batensoda from Geriyo Lake

Abstract

The comparison of haematological indices of Chrysicthys nigrodigtatus and Synodontis batensoda was carried out to evaluate their blood group, genotype and haematological profile. Test tube techniques based on agglutination test was used to evaluate the blood group, while the genotype was determined by haemoglobin electrophoresis. The haematological parameters were analyzed using standard methods. Sample size was n=10 for both fish. Results showed that WBC counts in C. nigrodigitatus males were 39.79±26.45μL and females 40.56±7.06μL, while RBC counts for males were 5.40±1.13μL and females 4.50±1.62mm3. Other results were: MCHC 33.31±0.01g/dL-1 and 33.29±0.05g/dL-1; PCV 33.6±6.73 and 33.80±9.44. HB 11.19±2.24 and 11.26±3.14 (g/dL-1); MCH 1.97±0.42 and 2.74±1.37μL and MCV 63.74±17.13 and 82.46±41.24 (fl) for male and female fish respectively. The genotype of all the males and females were the same (SS). The blood group recorded 40% for A+, 40% O+ and 20% for O- in males and 40% A+, 40% AB+ and 20% O- in females. Meanwhile, S. batensoda males and females had 10.16 ± 6.07μL and 10.65 ± 8.61μL in WBC and 9.14 ± 4.01μL and 7.76 ± 1.17μL in RBC counts respectively. While MCHC count was 33.32 ± 0.01 and 33.32 ± 0.00 g/dL-1; PCV count 39.00 ± 4.79 and 38.80 ± 5.21; HB count 12.99 ± 1.59 and 12.93 ± 1.73 (g/dL-1); MCH 1.64 ± 0.67 and 1.66 ± 0.10μL and MCV 49.64 ± 20.30 and 50.12 ± 3.11 (fl) all for males and females respectively. The genotypes of all the males and females were the same (SS). The blood group for males recorded 40% for A-, and 40% O+ and 20% O- while females recorded 40% A+, 40% O- and 20% for O+ respectively. This is similar to what is obtainable in humans. The differences in the blood group among the species could be associated with the differences in the physiological indices of the fish. Meanwhile the similar genotype observed in both species showed that the species were not migratory fishes but predominant in the lake.

Keywords: Haematological indices, blood group, genotype, Chrysicthys nigrodigtatus and Synodontis batensoda