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Jul 29, 2024Keywords:
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Main Article Content
Effect of glycerol on preservation of Mycoplasma capricolum subsp. capripneumonia (Mccp) in frozen lung Samples
Abstract
Mycoplasma capricolum subspecies capripneumoniae (Mccp) is the causative agent of contagious caprine pleuropneumonia (CCPP), a disease that affects the respiratory system of goats. Diagnosis of CCPP encounters challenges since Mccp is fastidious making it difficult to isolate or revive from stored samples. This study assessed the effect of glycerol on success of isolating Mccp from stored lung tissues. Experimentally infected goats developed fever and respiratory both consistent with CCPP and about 83% of the infected goats showed a variety of gross lesions including red hepatization, hyperemia, fibrinous pleural deposits and fibrinous adhesions. Three pieces of lung samples were collected from the same site of each of the 24 experimentally infected goats immediately after euthanasia. One piece was placed in media prepared with 8 weeks old serum immediately after collection for cultivation. The other two pieces were stored with and without glycerol at - 40℃. The stored samples were retrieved and cultured after 21, 34 and 39 months and the samples where Mccp was isolated re-cultured after six months. Mccp was not isolated in fresh lung samples. There was 50% (4/8) success in isolation of Mccp for lung samples stored in glycerol for 21 months compared to 37.5% (3/8) for those stored for 34 months. Out of the four lung samples which yielded Mccp at 21 months, Mccp was isolated in all four at 27 months, 3 samples at 34 months, and in no samples after 40 months of storage. At all-time points, samples stored without glycerol did not show growth. The findings demonstrate the effect of glycerol in preserving Mccp viability in longer duration periods storage. Further studies are needed to assess success of isolating Mccp using a single batch of quality-controlled serum, aliquoted and stored at different time intervals.
