In vitro efficacy of sweet orange peelings extract on egg hatchability and juvenile mortality of Meloidogyne javanica

Abstract

The effect of extract of Sweet Orange Peeling (SOP) was investigated on egg hatchability and juvenile mortality of Meloidogyne javanica in the laboratory. One hundred (100) eggs were introduced into each of 15 Petri dishes and 1000 juveniles in 15 Petri dishes each were independently exposed to crude extract (100%) concentration, crude + 5m1 distilled water crude + l0ml distilled water, crude + 15m1 distilled and distilled water only served as control. The Petri dishes were arranged in completely randomized design, percentage egg hatchability and mortality were calculated over l20 hours. The results indicated that crude extracts gave the least percentage of egg hatching with highest percentage of juvenile mortality. Other dilutions also significantly inhibited egg hatching and caused juvenile mortality when compared to the control. The results further revealed that hatching decreased with increase in duration of exposure and juvenile mortality increased with increase in exposure time. It could therefore be concluded that extracts were able to reduce egg hatching and cause juvenile mortality of M. javanica in the laboratory. However, further research in screen house and field is recommended to ascertain their efficacy.