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Étude de l’influence du chlorure mercurique sur la survie in vitro d’explants et de l’aptitude à la régénération de teck (Tectona grandis L. f., Verbenaceae)
Abstract
Objective: The teak tree (Tectona grandis L. f.) is an important woody plant in Benin. To avoid the difficulties
related to the propagation of this species, in vitro micropropagation tests on juvenile material of teak was
conducted. The objective of this study was to study the survival and regeneration behavior of two teak varieties
to allow their micropropagation in Benin. Methodology and results: Thus, the optimal concentration of mercuric chloride (HgCl2) and immersion time for adequate disinfection of explants of teak was determined. The variety which had the highest regeneration ability was identified. The results of these tests showed that a 0.1% concentration of the disinfectant (HgCl2) combined with an immersion time of 10 minutes provided optimal rate of survival of teak explants grown on MS nutrient medium diluted by half (MS / 2). Moreover, the local variety was found to be more efficient in regeneration on the same culture medium MS/2. Conclusion and application: This study revealed that the optimal dose of mercuric chloride (HgCl2) providing a high disinfection rate of teak explants was 0.1%. Besides, the hormonal balance between Benzylaminopurin and Naphtalen Acetic Acid did not generate a good rooting rate. These results indicate that valuables perspectives exist in term of teak micropropagation through in vitro tissue culture in Benin.
Keywords: Teak, variety, micropropagation, MS culture medium, mercuric chloride.
J. Appl. Biosci. 2013
related to the propagation of this species, in vitro micropropagation tests on juvenile material of teak was
conducted. The objective of this study was to study the survival and regeneration behavior of two teak varieties
to allow their micropropagation in Benin. Methodology and results: Thus, the optimal concentration of mercuric chloride (HgCl2) and immersion time for adequate disinfection of explants of teak was determined. The variety which had the highest regeneration ability was identified. The results of these tests showed that a 0.1% concentration of the disinfectant (HgCl2) combined with an immersion time of 10 minutes provided optimal rate of survival of teak explants grown on MS nutrient medium diluted by half (MS / 2). Moreover, the local variety was found to be more efficient in regeneration on the same culture medium MS/2. Conclusion and application: This study revealed that the optimal dose of mercuric chloride (HgCl2) providing a high disinfection rate of teak explants was 0.1%. Besides, the hormonal balance between Benzylaminopurin and Naphtalen Acetic Acid did not generate a good rooting rate. These results indicate that valuables perspectives exist in term of teak micropropagation through in vitro tissue culture in Benin.
Keywords: Teak, variety, micropropagation, MS culture medium, mercuric chloride.
J. Appl. Biosci. 2013
