Callus induction from epicotyl and hypocotyl explants of Parkia Biglobosa (Jacq.) Benth

Abstract

Epicotyl and hypocotyl explants of Parkia biglobosa (Locust bean) were cultured in vitro to investigate their callogenic capacity. Established cultures were obtained and maintained on MS medium supplemented with either 2,4-D or NAA, each of concentration range of 0.4 – 1.0 mg/L. In general, while higher concentrations of 0.8 and 1.0 mg/L NAA appeared to have favoured callus production from these explants, the same concentrations of 2, 4-D appeared to have caused inhibition. The incubation period has an additional effect on callus production with respect to the epicotyl explants.

It is concluded that both epicotyl and hypocotyl explants of Parkia biglobosa can serve as sources of callus production, a starting point for a range of in vitro studies including micropropagation and conservation of this endangered species.

Key Words: Callus, epicotyl, hypocotyl, Parkia biglobosa, auxin, micropropagation.

Ife Journal of Science Vol.6(2) 2004: 95-100