Potentials of microorganisms associated with plantain peels in the Lagos Metropolis for biodegradation and bioconversion

Abstract

The role of microbes in the degradation of plantain derived-wastes and their potential to produce  cellulolytic enzymes was assessed. Soil samples of decomposing waste piles were collected from two major plantain markets in the Lagos metropolis and analyzed for physicochemical properties, toxic heavy metal content and microbial populations. Findings revealed that the values of moisture content of the two soils varied between 7.27±0.04 and 8.06±0.19 %. M-12 site had the highest organic matter content of 6.89±0.14 %. A similar pattern was observed for nitrate, phosphate and chloride levels while some heavy metals were also detected in varying and 4 high amounts. The highest viable bacterial counts was 58.0±2.9 x 10 cfu/g at MU and there were no fungi at the 3 site whereas M-12 had a fungal count of 40.0±3.3 x 10 cfu/g. Out of the total of 34 isolates encountered, 8 isolates having maximum cellulase activities were selected for further studies by the primary screening technique. These test organisms were then evaluated by secondary screening for enzyme production. The test organisms were  phenotypically and biochemically characterized and identified as Klebsiella pneumoniae spp pneumoniae (2 strains), Klebsiella pneumoniae spp ozaenae, Enterobacter aerogenes, Providencia alcalifaciens, Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger respectively. Both the bacteria and moulds were found to be capable of utilizing lignin and cellulosic substrates for growth and for production of cellulolytic enzymes. It is suggested that such microorganisms could be useful in bioconversion of cellulosic substrates like plantain-derived wastes for biotechnological applications.

Keywords: Plantain derived-wastes, Soil, Heavy metals, Bacteria, Cellulolytic enzymes, Fungi.