Aldolase in concentrated ficoll 70 solution: analysis with Scaled particle theory

Abstract

The apparent average buoyant molar mass, M*_app , of Ficoll 70 and 2 x 10^-3 g/cm³ aldolase in Ficoll 70 weremeasured as a function of Ficoll 70 concentrations at concentration up to 0.16 g/cm³ in 0.15 M NaCl, pH 7.4, using tracer sedimentation equilibrium at 25^o C and 37^o C. The dependences were quantitatively accounted for by scaled particle theory of fluid mixtures using a simple model in which a weak attractive interaction between Ficoll 70 and aldolase was assumed, in addition to the excluded volume between the component mixtures. The self interaction between Ficoll 70 was repulsive and independent of temperature in the range of this experiment. The excluded volume between Ficoll 70 and aldolase was significantly reduced by the attractive interaction, which was dependent on temperature.

Keywords: Sedimentation Equilibrium, Crowder, FITC, Interaction, Aldolase, Ficoll 70