Free proline, soluble sugars and soluble proteins concentration as affected by salt stress in two sugarcane (Saccharum sp.) cultivars differing in their salt tolerance

Abstract

Salt stress is one of the environmental stresses which affect negatively the agricultural production of the world. In this study, the effects of salt stress on free proline, soluble sugars and soluble proteins accumulation were investigated in two sugarcane (Saccharum sp.) cultivars: CP66-346 (salt- tolerant) and CP65-357 (salt-sensitive). Young plants of these cultivars were exposed, in a hydroponic system, to four concentrations of NaCl (0, 17, 34 and 68 mM). These concentrations correspond to an electric conductivity of 1; 2.83; 4.26 and 6.63 mS/cm, respectively. Free proline, soluble sugars and proteins accumulation were quantified after 2 weeks of stress. Proline and soluble sugars concentrations increased significantly in leaves and roots under salinity. Furthermore, NaCl caused an increase in soluble proteins concentration in leaves and roots of the tolerant cultivar CP66-346 and a decrease in leaves and roots of the sensitive CP65-357. Salttolerant CP66-346 plants accumulated more soluble sugars in leaves than CP65-357, while both cultivars accumulated similar quantities of proline in leaves. These results provided evidence that soluble sugars could contribute mainly to counteract the negative water potential of the outside medium and that protein synthesis stimulation was implicated in sugarcane salt tolerance. Proline appeared as a symptom in salt-stressed sugarcane plants rather than as an indicator of tolerance.

Keywords: Sugarcane, Saccharum sp., proline, soluble sugars, soluble proteins, salt-tolerance