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Blood incubation infectivity test (BIIT): usefulness as compared to the SRAPCR in the identification of Trypanosoma brucei rhodesiense in animal reservoirs
Abstract
This investigation is aimed at comparing the Blood Incubation Infectivity Test (BIIT) with the Serum Resistance Associated gene Polymerase Chain Reaction (SRA-PCR) techniques as tools that can be used to
detect the presence of Trypanosoma brucei rhodesiense parasites in domestic animals reservoirs. Blood samples from 4754 animals (cattle, pigs and small ruminants) were screened for the presence of Trypanozoon
parasites by microscopy and in total, 109 samples (98 T. brucei sl isolates from domestic animal and 11 known T. b. rhodesiense) were analysed. There was 81.6% agreement between the results from the BIIT and the SRAPCR, with Cohen Kappa of 0.63 suggesting a substantial agreement between the two tests. When SRA-PCR was taken as the reference standard, the BIIT test had a sensitivity of 82.1% and specificity of 81.4%. The positive and negative predictive values were 74.4% and 87.3% respectively. Therefore, although the BIIT still remains a feasible test for detecting human infective trypanosomes in many African laboratories, SRA-PCR offers a quicker method for the identification of the animal reservoir for T. b. rhodesiense to enable Governments make firm decisions on public good investment on treating infected animals for the purpose of
controlling the zoonotic sleeping sickness.
detect the presence of Trypanosoma brucei rhodesiense parasites in domestic animals reservoirs. Blood samples from 4754 animals (cattle, pigs and small ruminants) were screened for the presence of Trypanozoon
parasites by microscopy and in total, 109 samples (98 T. brucei sl isolates from domestic animal and 11 known T. b. rhodesiense) were analysed. There was 81.6% agreement between the results from the BIIT and the SRAPCR, with Cohen Kappa of 0.63 suggesting a substantial agreement between the two tests. When SRA-PCR was taken as the reference standard, the BIIT test had a sensitivity of 82.1% and specificity of 81.4%. The positive and negative predictive values were 74.4% and 87.3% respectively. Therefore, although the BIIT still remains a feasible test for detecting human infective trypanosomes in many African laboratories, SRA-PCR offers a quicker method for the identification of the animal reservoir for T. b. rhodesiense to enable Governments make firm decisions on public good investment on treating infected animals for the purpose of
controlling the zoonotic sleeping sickness.
