Article Sidebar
Article Details
Issue
Section
Articles
Submission of a paper for publication implies the transfer of the copyright from the author(s) to the publisher upon acceptance. International Formulae Group is therefore the copyright holder after publication of an article in International Journal of Biological and Chemical Sciences, and published articles should not be used for commercial purpose without the written consent of the Editor-in-Chief. They are licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.
Main Article Content
Protein enrichment of Irish potatoes by fermentation process using mutant isolates of Latobacillus bulgaricus
Abstract
The study undertook the mutational selection of lactic acid bacteria used for the fermentation of peeled Solanum tuberosum (Irish Potatoes, Subtrate) in order to isolate mutants that may produce fermented Irish
potatoes product with improved nutritional quality (i.e increase protein content). Lactic acid bacteria were isolated from local yoghurt (“kindirmo” in hausa language) that were morphologically and culturally characterised and found to be predominantly Lactobacillus bulgaricus. Four (4) mutants selected randomly from Lactobacillus bulgaricus subjected to UV-light irradiation at 20 secs , 25 secs, 30 secs and 35 secs periods of exposure. The mutants exhibited similar morphological and cultural characteristics as the wild type strain. Fermentation of peeled Irish potatoes with wild type Lactobacillus bulgaricus gave no significant increase (P>0.05) in crude protein content from 7.37±0.42 g/100 g on the zero day to 8.73±0.43 g/100 g on the 5th day of fermentation, whereas, the mutant isolates gave a significant increase (P<0.05) of crude protein content from 7.37±0.42 g /100 g (zero day of fermentation) to 11.52±0.62 g/100 g, 12.18±0.62 g/100 g, 9.72±0.88 g/100 g and 14.50±0.52 g/100 g at 6th day of fermentation respectively. The mutant isolates, Lb35, showed the highest crude protein content (16.91±0.73 g/100 g) increase after the 5th day of fermentation of Irish Potatoes (Solanum tuberosum). The genetic analysis resealed that the Lactobacillus bulgaricus studied did not harbour any extrachromsomal element.
potatoes product with improved nutritional quality (i.e increase protein content). Lactic acid bacteria were isolated from local yoghurt (“kindirmo” in hausa language) that were morphologically and culturally characterised and found to be predominantly Lactobacillus bulgaricus. Four (4) mutants selected randomly from Lactobacillus bulgaricus subjected to UV-light irradiation at 20 secs , 25 secs, 30 secs and 35 secs periods of exposure. The mutants exhibited similar morphological and cultural characteristics as the wild type strain. Fermentation of peeled Irish potatoes with wild type Lactobacillus bulgaricus gave no significant increase (P>0.05) in crude protein content from 7.37±0.42 g/100 g on the zero day to 8.73±0.43 g/100 g on the 5th day of fermentation, whereas, the mutant isolates gave a significant increase (P<0.05) of crude protein content from 7.37±0.42 g /100 g (zero day of fermentation) to 11.52±0.62 g/100 g, 12.18±0.62 g/100 g, 9.72±0.88 g/100 g and 14.50±0.52 g/100 g at 6th day of fermentation respectively. The mutant isolates, Lb35, showed the highest crude protein content (16.91±0.73 g/100 g) increase after the 5th day of fermentation of Irish Potatoes (Solanum tuberosum). The genetic analysis resealed that the Lactobacillus bulgaricus studied did not harbour any extrachromsomal element.
