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Comparative efficacy of captopril and allylmercaptocaptopril in the maintenance of oxidative-antioxidative equilibrium and attenuation of selenite-induced cataract in rats
Abstract
The present study was undertaken to investigate the comparative efficacy of captopril (CP) and allylmercaptocaptopril (AMC) supplementation following selenite exposure with respect to oxidant-antioxidant
equilibrium and prevents cataract formation in rat pups. Forty Wistar-albino rat pups were equally divided into 4 groups. In Group I; only subcutaneous (s.c) saline was injected. In Group II; single dose of sodium selenite
(19 ìM/kg body weight, s.c) was injected on postpartum day 10. One day before the selenite challenge, the pups in Group III and IV were injected intraperitoneally a single dose of 50 mg/kg of CP and AMC
respectively and repeated once daily thereafter up to 21st day. The development of cataract was assessed weekly. The density of cataract was graded by slit-lamp biomicroscopy. On day 21, the blood was collected
and lenses were removed. Total oxidant status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI) and malondialdehyde (MDA) level were determined in the lenses of the rat pups. Serum paraoxonase-1
(PON1) activity was determined. All of the lenses of rat pups in Group I remained clean. All rat pups developed dense nuclear opacity in Group II. Three out of 10 rat pups developed slight nuclear opacity in
Group III while only one rat pup out of 10 in Group IV had vacuolization but not opacity. Differences among the groups were statistically significant (p<0.01). Group II lenses had higher mean OSI and MDA level than
those of Group III and IV (p<0.01). Group II lenses had lower mean TAC level and serum PON1 activity than those of Group III and IV (p<0.01). A negative correlation was found between serum PON1 activity and lens
MDA level (r = -0.6861; P<0.001). CP and AMC both diminished the incidence of cataract due to its protection of the antioxidant defense system but AMC was found to be more effective than CP.
equilibrium and prevents cataract formation in rat pups. Forty Wistar-albino rat pups were equally divided into 4 groups. In Group I; only subcutaneous (s.c) saline was injected. In Group II; single dose of sodium selenite
(19 ìM/kg body weight, s.c) was injected on postpartum day 10. One day before the selenite challenge, the pups in Group III and IV were injected intraperitoneally a single dose of 50 mg/kg of CP and AMC
respectively and repeated once daily thereafter up to 21st day. The development of cataract was assessed weekly. The density of cataract was graded by slit-lamp biomicroscopy. On day 21, the blood was collected
and lenses were removed. Total oxidant status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI) and malondialdehyde (MDA) level were determined in the lenses of the rat pups. Serum paraoxonase-1
(PON1) activity was determined. All of the lenses of rat pups in Group I remained clean. All rat pups developed dense nuclear opacity in Group II. Three out of 10 rat pups developed slight nuclear opacity in
Group III while only one rat pup out of 10 in Group IV had vacuolization but not opacity. Differences among the groups were statistically significant (p<0.01). Group II lenses had higher mean OSI and MDA level than
those of Group III and IV (p<0.01). Group II lenses had lower mean TAC level and serum PON1 activity than those of Group III and IV (p<0.01). A negative correlation was found between serum PON1 activity and lens
MDA level (r = -0.6861; P<0.001). CP and AMC both diminished the incidence of cataract due to its protection of the antioxidant defense system but AMC was found to be more effective than CP.
