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Performance of selective media for E. coli O157, using non-sorbitol fermenting strains and physiological characters of shiga-toxin producing species isolated in Côte d'Ivoire
Abstract
Performance of three selective media, tmperature and pH effects on growth of E. coli O157:H7 isolated in Ivory Coast were investigated. Thirty-eight (38) non-sorbitol fermenting E. coli were characterized by serotyping, detection of shiga-toxin gene (PCR) and plating on SMAC, VRMUG and BCMtm media for Performance evaluation. Temperature and pH effects on E. coli O157:H7 were measured in varied culture conditions. All culture media were sensitive (100%) for the growth of E. coli O157. Their specificities were 97.3, 94.7 and 50.7% respectively for VRMUG, BCMtm and SMAC media. Predictive value for VR-MUG medium was 66%, whereas that for SMAC medium was only 5.4%. Serotype O157:H7 E. coli population grew slightly at 42 °C and didn’t grow under acid conditions (pH 4.5, 3.5). The performance of the culture media evaluated
and the physiological data obtained for E. coli O157:H7 during this study would contribute to improve both isolation and identification methods of the pathovars.
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Keywords: E. coli O157H7, performance, culture medium, shiga-toxin, pH, temperature
