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Comparison of the amino acid sequences of lectins from the seeds of Dioclea reflexa and Canavalia ensiformis
Abstract
The amino acid sequence of a glucose/mannose specific lectin from the seeds of Dioclea reflexa (Dioclea reflexa agglutinin II, DRA-II) was determined by sequential Edman analyses of the intact subunit, and of the peptides derived from the protein by enzymatic digestion with trypsin. This sequence was found to be very similar to those of the lectins from other Dioclea species, Dioclea grandiflora
and Dioclea lehmanii, and also the lectin from Canavalia ensiformis (Con A). Comparison of these amino acid sequences showed that a high degree of homology exists among these proteins.
Keywords: Dioclea reflexa, Leguminosaea, Dioclea seeds, lectins, amino acids.
International Journal of Biological and Chemical Sciences Vol. 1 (3) 2007: pp.275-280
