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Influence of sugars, pre-treatment with acid and suspending medium on the interaction of Salmonella typhimurium and Salmonella enteritidis with concanavalin A.
Abstract
The influence of experimental variables on the interactions of Salmonella Typhimurium L1388 (ST) and Salmonella Enteritidis L1225 (SE) with concanavalin A (con-A) were assessed. At a con-A concentration of 78.1 µg ml-1, ST was agglutinated and SE was not. The con-A-induced agglutination of ST was completely inhibited by equal concentration of D-(+)-mannose, D-(+)-glucose, D-(+)-galactose, N-acetyl-D-glucosamine, trehalose, β-D-(+)-cellobiose and raffinose. Pre-treatment of the bacterial cells with acid did not alter the con-A reactivity of ST and SE. Change in the suspending medium from PBS to immunogold buffer did not alter the con-A reactivity of ST and SE. Interactions of lectins with microorganisms have great diagnostic clinical laboratory value in microbial typing, taxonomic classification and epidemiology.
© 2007 International Formulae Group. All rights reserved.
International Journal of Biological & Chemical Sciences Vol. 1 (1) 2007: pp. 67-70
