Protein profile of breeding discrepancies of African catfish Clarias gariepinus in aquaculture practices

Abstract

The protein analysis focused on sarcoplasmic muscle o f protein constituents to improve heterogenous growth of C. gariepinus. Ninety juveniles were graded and selected for the top 10%, medium 10% and bottom 10% fish which represented fast, moderate and slow growing groups. The protein sample extracts were prepared by homogenizing 150 mg of fish muscle and electrophoretic run in Sodium Dodecyl Sulphate (SDS) polyacrylamide gel. Eleven bands were found in large fish, eight bands in the medium fish and seven bands were detected in the small fish class. The 2^nd to 5^th protein band having molecular weight (MW) ranging from 116,200 to 70 KDa was detected across all fish class sizes. The 6^th and 7^th band present in the large fish sample class but absent in the other two classes, having MW 54.52 KDa and 54.21 KDa respectively are assigned to Desmin protein. The 1^st band was the heaviest (MW 118,600 KDa) band only found in the large sized fish. The results revealed that 1^st 6^th and 7^th bands are the distinguishing protein bands and these proteins have contributed to the heterozygosity nature of the large size class which are most suitable for broodstock selection for future artificial propagation practices.
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Keywords: Electrophoresis, molecular weight, broodstock, sarcoplasmic, heterozygosity