Bioactive compounds in the stem bark of Albizia coriaria (Welw. ex Oliver)

Abstract

Albizia coriaria was investigated for the bioactive compounds present in its stem bark. The plant was selected on the basis of its widespread use in traditional herbal medicine. Extraction of the plant material was done with ethyl acetate, methanol and water and the bioactivity of each extract was tested against  Pseudomonas aeruginosa and Escherichia coli. Separation and purification of the compounds in the most active (ethyl acetate) extract was done using a  combination of chromatographic techniques. The compounds were identified by 1D and 2D -¹H and ¹³C NMR techniques as well as Mass spectrometry (MS). Six compounds, namely: Lupeol (1), Lupenone (2), Betulinic acid (3), Acacic acid lactone (4), (+) – Catechin (5) and Benzyl alcohol (6) were identified and  characterized from the ethyl acetate extract. The results of the bioactivity tests carried out in this study indicated that A. coriaria has potential antimicrobial activity. Four of the characterized compounds (1, 2, 3 & 5) have a wide range of biological activity reported in literature. This justifies the use of this plant in traditional medicine and indicates a promising potential for the development of medicinal agents from A. coriaria stem bark.

Keywords: Biological activity, Lupeol, Lupenone, Betulinic acid, Acacic acid lactone, (+) – Catechin.