Tomato pomace powder ameliorated cisplatin-induced microanatomical alterations in brain of Wistar rats

Abstract

Cisplatin chemotherapy is associated with neurotoxicity. Oxidative stress has been associated with its pathogenesis. Tomato (Lycopersicon  esculentum) pomace powder (TPP) may be a preventive agent by virtue of its known antioxidant property. The possible protective role of TPP against cisplatin-induced alteration of the microanatomy of rat brain was investigated. Thirty rats were divided equally into five groups: control, propylene glycol, TPP, cisplatin (10 mg/kg i.p.); TPP 50  mg/kg plus cisplatin (10 mg/kg i.p.). All administrations were oral by gastric gavage given for 24 days except cisplatin which was given intraperitoneally on days 10 and 17. Rats were euthanized on the 25^th day of treatment. Blood parameters checked, neurobehavioural tests done and brain tissue were examined with regards to micro-anatomical parameters. Cisplatin caused a significant (p<0.05) reduction in lymphocytes. Neurobehavioural results showed that cisplatin caused a  reduction in line crossing, rearing and forelimb grip. Cisplatin caused histological alterations in the cerebellum, dentate gyrus and Cornu Ammonis3 (CA3).  Co-treatment of cisplatin with TTP ameliorated these haematological, neurobehavioural and histological alterations. In conclusion, Lycopersicon esculentum (as tomato pomace powder) demonstrated neuroprotection against cisplatin-induced alteration of microanatomy of rat cerebellum, dentate gyrus and Cornu  Ammonis3 of rat brain.

Keywords: Lycopersicon esculentum, cisplatin, neuroprotection, Cornu Ammonis3, dentate gyrus, cerebellum.