Acute oral toxicity and phytochemical study of "Diabenorme" and "Thuquinone" used to treat diabetes

Abstract

The aim of the study was conducted to search for phytochemicals and evaluate the acute oral toxicity in mice for aqueous extracts of "Diabenorme" and "Thuquinone" used in the treatment of diabetes. "Diabenorme" is a combination of two medicinal plants: Persea americana (Lauraceae) and Anacardium occidentale (Anacardiaceae) while "Thuquinone" is composed of Pycnanthus angolensis (Myristicaceae). Phytochemical analysis was done using standard methods and acute toxicity test (OCDE 423) was performed by a fixed dose procedure consist in administration of three doses of 300, 2000 and 5000 mg/kg body weight of "Diabenorme" and "Thuquinone". Phytochemical analysis showed that the different drugs contained polyphenols, flavonoids, catechin tannins, alkaloids and saponins. Concerning acute toxicity test, no sign of toxicity and mortality were observed during the experiment after limit test of 5000 mg/kg. Thus, there were no significant differences (Fischer test, P ˃ 0.05) in the body weights between the control and treated animals. These results show that the aqueous extracts of "Diabenorme" and "Thuquinone" are potentially safe for oral consumption at acute administration up to dose of 5000 mg/kg. Further investigation is needed to evaluate its sub-acute toxicity.

Keywords: Diabenorme, Thuquinone, Phytochemical screening, acute toxicity.