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Jun 5, 2014Keywords:
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Induction characteristics and optimization of production conditions of Xylanase by selected fungal species on lignocellulosic substrates
Abstract
Five xylanolytic fungi were isolated from saw dust samples collected from a saw mill dump in Ibadan, Nigeria. The effect of lignocellulosic substrates on the production of xylanases was studied in shake flasks. Trichoderma sp. Fd4 produced high yield of the enzyme when grown in 2% wheat bran. Similarly, xylan, lactose and maltose, used as carbon sources induced the production of high levels of xylanase. Trichoderma viride Fd18 produced 10.4 U/ml of xylanase using NH4Cl as nitrogen source, while NH4NO3 repressed enzyme production by Aspergillus ustus Fb2. The fungal isolates produced good amount of xylanase at 30-40 oC, pH 4.5-6.0, 7 days of incubation, initial spore size of 106 and in presence of NaCl and KCl in the basal medium.
Keywords: Xylanase, fungi, lignocellulosic waste, submerged fermentation, optimization
