Antioxidative properties of Mallotus oppositifolium decoction leaves extract using in vitro models

Abstract

In an attempt to explain the scientific basis for the medicinal benefits of Mallotus oppositifolium (Euphorbiaceae), the phytochemical constituent and the antioxidative properties of the aqueous decoction leaves extract of the plant versus standard Vitamin C were assessed. The phytochemical analysis (screening and quantitative assay), the 1,1- Diphenyl-2-picrylhydrazyl (DPPH), Nitric oxide, and hydroxyl radicalscavenging, the reducing power, ferrous reducing antioxidant power (FRAP) and the β carotene bleaching of the extract were performed using colorimetric or spectrophotometric methods. Qualitative screening of the extract showed the presence of alkaloids, flavonoids, steroids, phenolic compounds, tannins, phlobatannins, saponins, lipids, carbohydrates, mucilages, and proteins. The phytochemical quantitative assay of the decoction extract revealed that the plant depicted flavonols (38.278 mg.g^-1 catechin equivalent), tannins (23.13 mg.g^-1 tannic acid equivalent), phenolics (13.73 mg.g^-1 tannic acid content), flavonoids (10.62 mg.g^-1 quercetin equivalent), proanthocyanidins (2.53 mg.g^-1 quercetin equivalent) contents. Alkaloids and saponins content were 200 mg.g^-1 and 285 mg.g^-1, respectively. The ferric reducing antioxidant power (FRAP) of Mo extract and the IC₅₀ of vitamin C were 228±0.4 mg.mL^-1 and 592.0±0.8 mg.mL^-1 , respectively. The DPPH radicalscavenging properties of IC₅₀ of vitamin C was 480.0±0.8mg.mL^-1 and that of Mo extract was 360.0±0.8 mg.mL^-1. The IC₅₀ of Mo’s reduce abilities was 39.2±0.8 mg.mL^-1 and that of vitamin C was 320.0±0.8 mg.mL^-1.The IC₅₀ of the hydroxyl radical scavenging activity of Mo was 75.2±0,8 mg.mL^-1 whereas that of IC₅₀ of vitaminC was not defined. The nitric oxide radical scavenging activity of IC₅₀ of Mo was 121.6±0.8 mg.mL^-1 and that of the vitamin C was 130.4±0.8 mg.mL^-1, respectively. The β carotene bleanching gives the IC₅₀ of M. oppositifolium was 12,0±0,0 mg.mL^-1 and for the vitamin C was 16.0±0,0 mg.mL^-1. The decoction leaves of Mallotus oppositifolium (Mo) exhibited powerful antioxidative properties as vitamin C. The plant can be a source of antioxidative materials.

Keywords: Traditional medicine, phytochemical analysis, antioxidant