The effects of pain sensitivity behaviour on Swiss White Mice administrated with Chloroquine Phosphate

Abstract

This study evaluates the effects of Chloroquine phosphate on pain sensation in mice considering the fact that Chloroquine as s chemotherapic agent is known for its neurotoxicity effect. The mice were divided into three groups of 10 mice each. While group 1 as the control, 2 and 3 as the test groups and group 1 received 0.2ml physiological saline i.p. while test groups 2 and 3 received 10mg/k (human therapeutic dose) and 20ml/kg (pharmacological  dose) of Chloroquine respectively. The tail flick and formalin tests were used to assess pain sensation. In the tail flick test, the latency of tail flick in group 2 and 3 were significantly lower compared to the control group in both phases, thus, showing an increase in pain sensation. In formalin tests, the frequency of right hind paw lick in group 2 was significantly higher compared to the control, representing an increase in pain sensitivity. The duration of hind paw lick was not significantly different among the groups. However, in phase 2, the duration of hind paw lick in group 2 was higher than control, showing an increase in chronic pain sensitivity. Our results suggest that, Chloroquine phosphate increases pain sensation in mice.

Keywords: Neurotoxicity, Chloroquine phosphate, pain sensation, tail flick tests, formalin tests.