In vitro activity of Allium sativum and Aloe vera extract against Salmonella enterica subspecies enterica serovar Gallinarum

Abstract

The rapid increase of antibiotic resistance needs to be taken as a threat to both animals and human being. In this study the arbitrary concentration of 25%, 50% and 75% of individual and combined Allium sativum and Aloe vera were tested against Salmonella Gallinarum. The antimicrobials were extracted using aqueous solution where 25g, 50g and 75g of Allium sativum were dissolved in 100ml of sterile distilled water and left for 24hrs and filtered to obtain the concentration of 25%, 50% and 75% respectively and 1ml, 2ml and 3ml of Aloe vera exudates were homogenized in 4ml of distilled water separately by centrifugation to obtain concentration of 25%, 50% and 75%
respectively. Aqueous extract of Allium sativum and Aloe vera exudates of 25%, 50%, 75% concentration and 25ml, 50ml, 75ml respectively were homogenized together to obtain a mixture. The different concentration of single and mixtures were tested against Salmonella Gallinarum for growth inhibition through pour plate method. The concentration of 50% and 75% illustrated strong antimicrobial activities but Allium sativum was more effective than Aloe vera. Combined concentration of 25% was less effective but those of 50% and 75% were highly effective than corresponding concentration of single. The combined concentration at 50% and 75% could be used to treat or to control clinical cases in poultry production caused by Salmonella Gallinarum with low cost compared to the synthetic drugs which have become less effective and have high cost. Aloe vera and Allium sativum can be refined and processed in the pharmaceutical industries at required concentrations and sold to the farmers at low cost for inhibition and control of Salmonella Gallinarum in poultry production.