Evaluation Of Phytochemical Active Ingredients Present In Organic Solvent Extracts And Larvicidal Properties Of Some Selected Plants From Taraba State Against Anopheles Larvae

Abstract

The Continuous use of synthetic insecticides and its toxicity problems coupled with the incidence of insect resistance calls for the need for alternative insecticide. Plants extracts a rich source of bioactive compounds can provide an alternative source of insecticide which are ecofriendly. The research evaluated the phytochemical active ingredients present in organic solvent extracts and larvicidal effect of some selected plants from Taraba state against Anopheles larvae. World Health Organisation protocol was adopted for the larvicidal bioassay. Twenty group of fourth instar Anopheles larvae were exposed to various concentrations of 200,400,600 and 800ppm, mortality was observed and recorded after 24 hours. The results of preliminary qualitative phytochemical analyses of tested plants revealed the presence of some secondary metabolite that may be responsible for the bio control potentials. Acetone extract of Hyptis suaveoleons against the fourth instant anopheles larval was observed to have the highest percentage mortality among the entire plants extracts than the aqueous. The lowest mortality was observed at 200ppm with 35.0% while the maximum was at 800ppm with 80.0%. Lc₅₀ and Lc₉₀ values were achieved at 438 and 866ppm with the LCL -UCL 340-540 and 722-1151 respectively. While the biological activity of Hyptis suaveolence aqueous extract had its lowest mortality at 200ppm with 36.67% while the maximum was at 800ppm with 80.0%. Lc₅₀ and Lc₉₀ values were achieved at 582 and 1225ppm with the LCL -UCL 449-806 and 942-2636 respectively. Ancova results showed no significance difference at p>0.05 among the mean percentage mortality of the treated doses.