Biochemical implication of administration ofmethanol extract of Ocimum gratissimum leaf on haematological profile of Wistar rats

Abstract

In the search for medicinal plants that will provide ameliorative measure to patients with anaemic disorders. The methanol extract of O. gratissimum leaf was administered to Wistar rats for its effect on haematological profile. Twenty-eight (28) male Wistar rats ranging from 180-200g was randomly picked and placed into plastic cages labeled A-D. Group A served as the control group while groups B-D was the test groups. The  animals in group A was administered with distilled water orally by gavage. Group B were administered 50mg/ kg body weight of methanol extract of O. gratissimum, group C were administered with 100mg/kg bodyweight while group D were administered with 200mg/body of methanol extract of O. gratissimum for 14 days. Blood was collected from all the test rats and control by cardiac puncture using disposable syringe and needle then dispensed into tubes containing EDTA. The extract displayed a significant increase (p<0.05) in RBCs, Hb, PCV and platelet counts. More so, the  extract produced no significant (p>0.05)difference in MCV, MCH, MCHC and RDW WBC counts when compared with the normal control .Therefore, it will be logical to conclude that the extract of O. gratissimum might be a panacea in the management of anaemic conditions when properly harnessed due to its erythropoietic, haematopoietic and thrombopoietic effect.

Keywords: Anaemia, erythropoietic, haematopoietic, thrombopoietic and phytochemicals.