Comparative evaluation of the efficacy of Pseudomonas putida in the bioremediation of diesel fuel contaminated derno-podzoluivisolic soil of different horizons

Abstract

This study was carried out to compare the efficacy of Рseudomonas putida, contained in the biopreparation «Pseudomin» in the bioremediation of diesel fuel contaminated derno-podzoluivisolic soil of two different horizons. By analyzing the Total Petroleum Hydrocarbons (TPH) content using IR-spectrometry method under laboratory conditions, results of the research showed that the inoculation of Рseudomonas putida in to the diesel fuel contaminated derno-podzoluivisolic soil samples in only 12 weeks was able to reduce the diesel fuel content up to 99.2% in the humus-accumulative horizon; and up to 92.8% in the eluvial horizon. Comparative evaluation of the contaminated variants inoculated with Pseudomonas of both horizons revealed a significantly higher rate of diesel fuel degradation in the humus accumulative horizon of up to 8.7 times, compared to the eluvial horizon, which shows a slightly slower rate of degradation.

Keywords: Bioremediation, petroleum hydrocarbon, humus-accumulative horizon, eluvial horizon, dernopodzoluivisolic soil, Рseudomonas putida, biological preparation «Pseudomin»