Effect of applied pressure on callus formation and its relevance in grafting

Abstract

Studies were carried out on Citrus limon explants in vitro to determine the effect of applied pressure on callus formation, cell differentiation and its importance in grafting. Callus was formed earlier in pressure-free explants. The importance of applied pressure in graft formation was indicated by the fusion of callus cells in capped explant experiments. Where callus grew from the edges, a cambium eventually formed within it from the old cambium. The new cambium always curved towards the phloem side, suggesting that a nutrient gradient may be responsible for its location.



Ghana Jnl agric Sci. Vol.32(2) 1999: 145-151