Green Synthesis for the Production of Glucose Syrup from Waste Cassava Starch Using Alpha-Amylase

Abstract

Research on production of glucose syrup from waste cassava starch has not been adequately covered. In this paper, we report on the process for breaking down starch through harnessing the catalytic properties of silver nanoparticles (AgNPs) in an enzymatic procedure that encompasses the breakdown of starch by alpha-amylase. The analysis carried out on the starch-synthesized AgNPs had its peak absorbance in the region between 400 nm and 500 nm, the X-ray diffraction (XRD) result also showed that the AgNPs were crystalline and this indicated that the nanoparticle formed could be used for hydrolysis. The presence of silver nanoparticles was observed to enhance the speed at which starch was enzymatically broken down as sample G1 (which was the sample with an equal amount of silver nanoparticle and alpha-amylase) has a concentration of 5.23 mg/mL (which implies a percentage conversion of approximately 52.3%). It is hypothesized that alpha-amylase became immobilized on the nanoparticle surface giving rise to a better glucose yield in this study.