Prevalence, risk factors and antibiogram of Escherichia coli isolated from dogs in Ambo, Gojo and Bako towns of Oromia region, Ethiopia

Abstract

Dogs are a potential reservoir for Escherichia coli and other zoonotic bacterial pathogens posing the risk of infection to humans and other animals. A crosssectional study was used to collect 438 rectal swab samples from apparently healthy dogs of Ambo, Gojo, and Bako towns of West Shewa Zone with the objectives of investigating the prevalence, risk factors and antibiogram of E. coli. A questionnaire survey was administered at the household level to collect data on potential risk factors to acquire E. coli infection. Prevalence was determined by using standard bacteriological culture techniques. Further  confirmation and antimicrobial susceptibility testing of E. coli using the minimum inhibitory concentration (MIC) method were  conducted using Automated Phoenix Machine. The overall prevalence of E. coli was found to be 24.2% (95% confidence interval [CI]:20.26-28.49%). The isolation rate of E. coli was the highest in Bako 31.01%, followed by Gojo 30.99% and Ambo 18.49% towns.  Univariable and multivariable logistic regression analyses revealed that there was a significant association between E. coli prevalence and towns and Kebeles (P<0.05). However, age, sex, the presence of other domestic animals in the household, types of the housing system, educational level of the dogs’ owners, and type of feed provided to the dogs did not significantly correlate with the isolation of E. coli (P > 0.05). E. coli isolates were pan-susceptible to amikacin, ertapenem, imipenem, piperacillin-tazobactam, netilmicin, ampicillin, piperacillin, cefoxitin, ciprofloxacin, levofloxacin, and meropenem. A low level of resistance was found to aztreonam, ceftriaxone, (each 13.95%), cefazolin (22.73%), gentamicin (11.90%), and trimethoprim-sulphamethoxazole (6.97%). E. coli isolates showed multidrug resistance to aztreonam, cefazolin, ceftriaxone, gentamicin, and trimethoprim-sulphamethoxazole (14.0%). This demonstrated E. coli prevalence and moderately low antimicrobial resistance  suggests the possible risk of infection of humans. Therefore, raising public awareness about zoonotic canine disease prevention measures and good hygienic practices are essential..

Keywords: Antibiogram, Dog, Escherichia coli, Ethiopia, Prevalence, Risk factor