In-vitro antioxidant and anti-inflammatory activity of Annona muricata leaf extract mediated silver nanoparticles

Abstract

The reactive oxygen species produced by the action of free radicals on molecular oxygen causes serious inflammation conditions. Non-steroidal anti-inflammatory drugs which are commonly prescribed for the treatment of inflammatory conditions are associated with side effects. Therefore, silver nanoparticles synthesized using Annona muricata leaf extract as a reducing agent was investigated for its in-vitro antioxidant and anti-inflammatory potentials. Aqueous extract of Annona muricata leaf was used to prepare silver nanoparticles using AgNO₃. The structure and surface morphology of the silver nanoparticles was characterized using UV-VIS spectrophotometer, scanning electron microscope (SEM), energy dispersed X-ray (EDX) and X-ray diffraction spectroscopy. SEM images confirmed that the particles were spherical in shape. The EDX showed that silver was the most abundant element in the samples. The biosynthesized silver nanoparticles showed low DPPH and ABTS antioxidant activities compared with the standard but its activity in scavenging hydrogen peroxide was high at 20 – 100 μg/mL. Also, the silver nanoparticles showed high anti-inflammatory activity on lypoxygenase inhibition, proteinase inhibition and red blood cell membrane stabilization. From the data obtained from the various analysis, it can be concluded that the silver nanoparticles synthesised with Annoma muriata leaf extract is efficient as antioxidant and anti-inflammatory agent which can serve as a suitable alternative to expensive products.