The role of cytokinin and auxin in organogenesis of Passiflora mollissima and evaluation of biochemical changes using isozyme and protein profiles

Abstract

Passiflora mollissima (H.B.K) Bailey is a climber noted specially for its ornamental and medicinal property. It has been micropropagated successfully by culturing nodal and internodal segments. Murashige and Skoog's basal medium with 3% sucrose and augmented with 6-benzyl amino purine at 4.44 μm showed the maximum number (12.5 ± 0.25) of shoot p oiiferation from the nodal segments. Maximum percentage (82 ± 0.02) of shoot proliferation was also achieved on Murashige and Skoog's basal medium with 3% sucrose and fortified with 4.44 μm 6- benzyl amino purine. Maximum percentage (78.7 ± 0.5) of callus formation was achieved on Murashige and Skoog's basal medium supplemented with 3% sucrose and 4.52 μm 2. 4-dich lorophenoxy acetic acid. Maximum percentage (78 ± 0.02) of shoot proliferation from the internodal derived calli was observed on Murashige and Skoog's medium augmented with 3% sucrose and 4.44 μm 6-qenzyl amino purine in combination with 2.69 μm naphthalene acetic acid. The in vitro raised shootlets were transferred to half strength Murashige and Skoog's medium supplemented with auxin for rooting. Maximum frequency (83 ± 0.1) of rootlets and maximum number of rootlets (9.2 ± 0.01) per shootlet formation were obtained in half strength Murashige and Skoog's medium fortified with 4.92 μm indole 3-butyric acid. The micropropagated plantlets' genetic uniformity was confirmed through the isozyme and protein profiles. Electrophoretic studies confirmed the genetic similarity between the mother plant and the plant derived from nodal segments; the genetic variation was noticed in calli-mediated plants. The in vitro raised plants were hardened in poly-cups. About 80% of plants were established well in the poly-cup stage, and 75% of giants succeeded in the field establishment.

Keywords: Passiflora mollissima, In vitro shoot proliferaition, Callus.