Chemical analysis of propolis from different regions of Egypt

Abstract

Propolis samples were extracted from beehives using sharp knife the conventional method in the apiaries located in the Governorates of Sohag, Beheira, Qalubia, Ismailia, and Zagazig. Additionally, two varieties of bee propolis were collected using traps in this investigation as follows: Hand-cut fiber screens with circular holes in a diameter of 1 mm., Fiber mesh sheets (45*35 cm) were put onto the top bar of  the combs. Hand - cut polypropylene plastic screens with round holes in diameter of 2mm. Plastic mesh sheets (45*35 cm) were set onto  the top bar of the combs inside the honeybee hive presented. Eighty percent methanol was used to extract the samples. Bee propolis was  analyzed using LC-MS spectral database, displayed the composition scrape that follows for each Governorate Sohag: ester or ether  of tamarixetin - syringic, caffeic-hexose-tartaric, caffeic-quinic acids, gallic-vanillin and acetyl gallate. In Beheira: quinic-hexose, ferulic- hexose, synapticpentose or apigenin or one of its isomer malonate, apigenin and vanillic acid. In Qalubia: myricetin, coumaric-hexose- malonate, ferulic-hexose, synaptic pentose or apigenin or one of its isomer malonate. In Ismailia: ferulic-hexose, synaptic-pentose or  apigenin or one of its isomer malonate. In Zagazig: Cyanidin, pelargonidin or apigeninidin with benzoic, hydroxyl benzoic. Based on the  results of analysis the biological effect of each separated component has been studied.