Ameliorative Effect of Parsley and Pumpkin Seeds Oils against Cosplatin Induced Hepatorenal Injury in Male Albino Rats (Physiological and Histological Study)

Abstract

Background: Cisplatin is a widely used chemotherapeutic agent with significant hepatotoxic and nephrotoxic side effects.

Objective: This study aimed to investigate the protective effects of parsley oil (PO) and pumpkin seed oil (PSO) against cisplatin-induced organ toxicity in male albino rats.

Methods: A batch of male albino rats weighing between 160 and 200 grams was divided into four groups. Group 1 functioned as the  control group. Group 2 was administered a solitary dose of cisplatin at a dosage of 7.5 mg per kilogram of body weight, intraperitoneally.  Groups 3 and 4 were pre-treated with PO or PSO (0.1 ml/kg BW, orally) for ten days and then received cisplatin on the sixth day. On day  11, blood samples were obtained for biochemical examination. Histopathological examination of kidney samples was also conducted.

Results: Cisplatin administration significantly elevated ALT, AST, urea, and creatinine levels, indicating liver and kidney damage. Pre- treatment with PO and PSO significantly mitigated these effects, normalizing the biochemical markers. Histopathological analysis  supported these findings, showing reduced tissue damage in PO and PSO groups.

Conclusion: Parsley oil and pumpkin seed oil provided  defense against liver and kidney damage caused by cisplatin in rats, suggesting their potential as adjuncts in chemotherapy