Extraction, Isolation and Identification of Some Natural Bioactive Marine Products from <i>Squilla mantis</i> (Stomatopoda-Squillidae) with Special Reference to Seasonal Variation of Marine Algae diversity

Abstract

Background: Marine algae important in food chain, productivity and marine ecosystem, Also Squilla mantis is healthy human food. Aim of the work: To identify some biologically active natural marine products, and screening its bioactivity, as well as discover the seasonal variation diversity of associated marine algae, for its role in the marine food chain.
Materials and methods: The squills were collected and marine macroalgae associated with it. Also, some biologically active marine natural products were extracted and identified from shell extract to examine its effect on some microbs and laryngeal cancer.
Results: The results showed a seasonal variation in diversity of marine algae, which indicated the effect of seasonal change food availability and environmental yield. The results of antimicrobial activity of Squilla mantis shell extract indicated an important effect differences between the groups treated with shell extract (1.5, 2.5, and 5 mg/mL) compared to the control . Also, evaluation of viability of Hep-2 cell line post treatment with shell extract of Squilla mantis using sufranin uptake assay as (MTT) uptake and microscopic examination indicated a significant difference between the groups treated with shell extract compared to the control group. Cell viability decreased depending on dose or concentration. The shell extract inhibited the proliferation of a larynx cancer cell line in a dose dependent manner.
Conclusion: The present study showed that, there are seasonal variation in marine algae diversity , which affect on productivity and food availability. Also, there are importance natural bioactive marine products from Squilla mantis shell extract .