Production Of Vitamin B12 By Streptomyces Fulvissimus

Abstract

Fifty five of actinomycete isolates were screened for vitamin B₁₂ production by growing on soybean meal medium fortified with cobalt. Only one AZ-Z-88 among nine actinomycete cultures was found to produce significantly higher yield of the vitamin B₁₂ (64.57 ug/ml). Determination of vitamin B₁₂ production was carried out using E.coli, ATCC 14169 as the test organism. The actinomycete culture could be isolated from a soil sample collected from zagazig city. Taxonomical features of the most producer isolate were matches with Streptomyces fulvissimus in its morphological, physiological and biochemical characters, therefore, it was given the suggested name Streptomyces fulvissimus, AZ-Z-88. The fermentation broth filtrates were extracted by using Chloroform. Purification of the active ingredient up to crystalloid form was performed by using both thin layer chromatography (TLC) and column chromatography technique. The spectroscopic analysis (UV, IR and HPLC spectrum) were used for comparative studies between vitamin B₁₂ produced by Streptomyces fulvissimus, AZ-Z-88 and standard vitamin Bv. Recorded data emphasized the fact that, the purified compound was suggestive of being belonging to vitamin B₁₂.

Egyptian Journal of Biomedical Sciences Vol. 23 (1) 2007: pp. 166-184