Single cell protein extraction from orange wastes using Aspergillus niger isolate no. 5 under solid state fermentation

Abstract

An attempt was made to optimize protein extraction from sweet orange wastes (SOW) and orange pulp wastes (OPW) using Aspergillus niger isolate No. 5 by solid state fermentation. Maximum protein enrichment of 52.48% and 46.50% were made using the substrates of SOW and OPW, respectively, on the seventh day of incubation at 30°C. The single cell protein quantified from the fungus grown on malt extract broth enriched with SOW and OPW were 23.14% and 21.35%, respectively; whereas the protein extracted from the growth of the fungus on potato dextrose culture broth enriched with SOW and OPW were 23.60% and 21.48%, respectively. The highest production of intracellular protein (70.46%) was obtained from OPW in comparison with that of SOW in soluble protein as intracellular protein (65.31%). It was clearly indicated that the standardization and determination method of protein using SOW and OPW wastes by A. niger isolate No. 5 has produced the maximum protein enrichment under solid state fermentation.

Key words/phrases: Aspergillus niger isolate, Orange wastes, Protein enrichment, Standardization of protein, Solid state fermentation.