Possible mechanism of disintegrin/like domain in mesenchymal stem cells homing in mice liver injury model

Abstract

Liver disease is one of the major health problems in many countries. Mesenchymal Stem cells have opened a new approach to deal with liver fibrosis. Improvement of bone marrow derived mesenchymal stem cellshoming after systemic injection of disintegrin/like domain purified from Egyptian horned Cerastes cerastes crude venomwhichwas shown byprevious study. This work was designed to investigate the possible mechanism of disintegrin/like domainin mesenchymal stem cells homing to fibrotic liver of CCl₄ treated mice. Quantitative gene expression was performed for stromal cell-derived factor-1α to study homing of bone marrow derived mesenchymal stem cells, transforming growth factor beta 1 to study bone marrow derived mesenchymal stem cellshoming and regeneration of liver cells and hepatocyte growth factor to study regeneration of liver cells in CCl₄ induced liver injury in white albino mice. It was found that disintegrin/like domaincould increase homing ofbone marrow derived mesenchymal stem cells as showed by the significant increase in liver stromal cell-derived factor -1α gene expression, transforming growth factor beta 1 as well as hepatocyte growth factor genes expression compared to the non disintegrin/like domaintreated CCl₄ group.

Keywords: BM-MSCs; CCl₄; HGF; SDF-1α; TGF-β1.