Chemokine receptor 2 (CCR2) G190A polymorphism in chronic renal failure patients requiring hemodialysis

Abstract

End-stage renal disease is associated with the inflammatory state characterized by infiltrating macrophages/lymphocytes, a major source of chemokines. The aim of this study was to determine the association of CCR2 G190A polymorphism in patients with chronic renal failure (CRF) requiring hemodialysis. Seventy CRF patients and thirty healthy controls were enrolled in the current study; PCR-RFLP technique was used to assess the gene frequencies of CCR2 G190A. The results of the present study showed that there was a significant difference in the genotype and allele frequency distribution of CCR2 G190A in CRF patients and control subjects. A significant association was found between CCR2 G190A and CRF risk, especially, the AA genotype (OR= 2.5, 95% CI=0.26-23.66) and A allele (OR=2.9, 95% CI=1.14-7.3). The polymorphism was significantly associated with the presence of diabetes mellitus. From this study, it could be concluded that, a significant association was found between the AA genotype of CCR2 G190A polymorphism and CRF. Other chemokine polymorphisms in renal pathologies have to be further investigated with larger population based studies.

Key words: CCR2, polymorphism, PCR-RFLP, CRF.